GHK-Cu reconstitution calculator
Reconstituting GHK-Cu
GHK-Cu comes in much larger fills than most peptides, so its solutions are strong. A GHK-Cu 50mg vial with 2 mL of bacteriostatic water gives 25 mg per mL, and the 100mg vial with 3 mL gives about 33 mg per mL. At those strengths each unit on a U-100 syringe holds a quarter of a milligram or more, so small amounts take only a few units.
The solution should be clear and blue; the colour comes from the copper and is expected. For GHK-Cu with water and a syringe in one carton, see the GHK-Cu Starter Kit.
GHK-Cu reconstitution chart
Concentration for each vial size and water volume. On a U-100 insulin syringe, 10 units is 0.1 mL and 100 units is 1 mL.
| Vial | Bac water added | Concentration | Per 10 units (0.1 mL) | Per 1 unit |
|---|---|---|---|---|
| 50 mg | 2 mL | 25 mg/mL | 2.5 mg (2500 mcg) | 250 mcg |
| 50 mg | 3 mL | 16.667 mg/mL | 1.667 mg (1666.667 mcg) | 166.667 mcg |
| 100 mg | 2 mL | 50 mg/mL | 5 mg (5000 mcg) | 500 mcg |
| 100 mg | 3 mL | 33.333 mg/mL | 3.333 mg (3333.333 mcg) | 333.333 mcg |
The formula behind the calculator
concentration (mg/mL) = GHK-Cu in vial (mg) ÷ water added (mL)
units on a U-100 syringe = amount (mg) ÷ concentration (mg/mL) × 100
Amounts in micrograms are divided by 1,000 first. The calculator does this arithmetic only; it does not recommend an amount, and GHK-Cu from Chainwell Peptide is supplied for laboratory research use only.
Other peptide calculators
Calculators for other peptides: Retatrutide calculator, BPC-157 calculator, BPC-157 and TB-500 Blend calculator, TB-500 calculator, CJC-1295 and Ipamorelin calculator, Tesamorelin calculator, MOTS-C calculator, PT-141 calculator. The main peptide calculator covers every vial in our range, and the reconstitution guide explains the technique step by step.
GHK-Cu calculator questions
How much bac water for 50mg GHK-Cu?
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2 to 3 mL in a standard 3 mL vial. 2 mL gives 25 mg per mL and 3 mL gives about 16.7 mg per mL. More water spreads the peptide over more units, which makes small amounts easier to measure.
How much bac water for 100mg GHK-Cu?
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Up to about 3 mL, the capacity of a standard vial, which gives about 33 mg per mL. Each unit on a U-100 syringe then holds about 0.33 mg.
Why is reconstituted GHK-Cu blue?
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The copper ion bound to the peptide gives the solution its blue colour. A clear, blue solution is normal; cloudiness or particles are not.


